麻杏石甘汤抗流感病毒有效部位黄酮含量测定

摘要：  目的  建立麻杏石甘汤抗流感病毒有效部位群总黄酮含量的测定方法。方法 芦丁参照品和样品分别经过Al2(NO3)3-NaOH-NaNO2显色，采用紫外分光光度法在333nm波长处测定麻杏石甘汤总黄酮含量。结果  回归方程为Y=0.01417X-0.01763，相关系数为0.99918；精密度高,重现性与稳定性较好。 结论  应用紫外分光光度法测定麻杏石甘汤总黄酮含量准确度较高，是切实可行的含量测定方法。
关键词：麻杏石甘汤；黄酮；Al2(NO3)3-NaOH-NaNO2显色法；紫外分光光度法 [资料来源：http://doc163.com]

Experimenal study Of Maxingshigan Valid Part flavonoids its Polysaccharides on anti-Influenza
Abstract:Objective  To establish the Method for Determination of Total Flavonoids in the Flavonoid Fraction of Maxingshigan dectoctin Antiviral Components. Methods  The content of total flavonoids was determined with Al2(NO3)3-NaOH-NaNO2 reaction development process,and selected rutin as reference substance. Results  The regression equation was as follows: Y=0.01417X-0.01763 (R2=0.99918);high précision preferably repeatability and stability. Conclusion  To determine content of flavonoids in rutin with ultraviolet spectrophotometry by standard Liquiritin is a practical method with higher accuracy.
Key words: Maxingshigan dectoctin; flavonoid; Al2(NO3)3-NaOH-NaNO2 reaction development process; ultraviolet spectrophotometry [资料来源：http://www.doc163.com]

目  录   7200字
摘要：    1
关键词：    1
1 前言    1
1.1 研究麻杏石甘汤抗流感病毒总黄酮含量测定意义    2
1.2   麻杏石甘汤提取精制工艺    2
1.3   黄酮的分离纯化工艺    3
1.4   黄酮的检测方法    4
2  实验部分    5
2.1  实验材料与仪器    5
2.1.1 实验材料(包括药品、试剂、材料)    5
2.1.2  实验仪器    5
2.2  实验步骤    5
2.2.1  麻杏石甘汤的制备    6
2.2.2  pH值梯度萃取法分离黄酮    6
2.2.3  紫外分光光度法测定总黄酮的含量    6
2.2.4  样品的测定    7
2.2.5  阴性样品的测定    7
3  讨论    8
参考文献：    8